Formation of polydeoxyadenylylthmidylate after deoxyribonucleic acid synthesis by a deoxyribonucleic acid polymerase from Mycobacterium smegmatis
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چکیده
منابع مشابه
Heterogeneity of deoxyribonucleic acid molecules isolated from Mycobacterium smegmatis.
Bulk chromosomal deoxyribonucleic acids (DNAs) of Mycobacterium smegmatis strains 607+ (wild type) and 607-1 (Strr) and orange-red pigmented variants (OR) were separated into two distinct bands (types 1 and 2) by cesium chloride density gradient centrifugation. Thermal denaturation analyses showed that type 1 and 2 DNA fragments of these strains possessed guanine plus cytosine contents averagin...
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MISSOURI The enzymatic synthesis of DNA1 in sufficient excess over the DNA used to prime the reaction21 a has enabled us to examine the chemical composition of the synthetic product. As has already been demonstrated, this DNA consists of deoxy-nucleotides Linked by typical 3'-5' phosphodiester bonds, and all four of the deoxy
متن کاملRole of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.
Exonuclease activity has been measured in preparations of both enzymes. The activity associated with DNA polymerase a requires denatured DNA, Mg2+ and is ATP-dependent. The polymerase b-associated nuclease requires denatured DNA and Mg2+ or Caz+. Studies of the distribution of these enzymes during the cell cycle have shown that extracts of cells harvested at the time of nuclear DNA synthesis (C...
متن کاملDeoxyribonucleic Acid polymerase from wheat embryos.
A soluble DNA-dependent DNA polymerase was extracted from wheat embryos. In vitro, the incorporation of radioactive thymidine triphosphate into acid-insoluble material is dependent upon the presence of the enzyme, all four deoxyribonucleotide triphosphates, Mg(2+), and a DNA template. Incorporation occurs on native, alkali-denatured, and strictly double-stranded DNA. The in vitro synthesized pr...
متن کاملEnzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.
Double stranded DNA serves as a template primer for Escherichia coli DNA polymerase when the DNA contains a single strand break (a nick) with a 3’-hydroxyl terminus. Initiation of replication entails covalent extension of the 3’-hydroxyl terminus and a concurrent 5’ + 3’ nuclease action by the enzyme. The primer strand is hydrolyzed at the 5’ side of the nick while the synthetic activity cataly...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1250101pb